an Identification of endophytic fungi associated with plants from the Alagoas Semiarid
DOI:
https://doi.org/10.48017/dj.v9i2.2859Keywords:
micro cultivation, riparian forests, filamentous fungiAbstract
In the semi-arid region of Alagoas, there are difficulties for small producers to provide food for animal production, water scarcity is one of the challenges faced and causes significant damage. However, lack of conservation and lack of knowledge are factors that attribute to overheating and lack of rainfall. By combining these factors, there is a need to study and prove the importance of these tree species not only in maintaining natural ecosystems, but also in improving the quality of animal production. Through the micro cultivation method, the primary identification of the endophytes was carried out, according to the Anvisa Manual (2004) for the identification of filamentous fungi. Morphological and microscopic aspects and sporulation patterns were observed. In sterile Petri dishes were slided with APD agar blocks. After sowing the endophytes in the culture media, they were covered with coverslips, after growth in 3 and 5 days, the coverslips were stained with methylene blue and, based on observation under a microscope, the sporulation structures were consulted by means of the bibliographical references such as atlases and manuals for identifying the genus of endophytes.
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